The 32-year-old is accused of raping two women, as well as sexually assaulting a third woman.
The alleged offences took place between 2021 and 2022, when he was an Arsenal player.
Partey spoke only to confirm his name, date of birth and his not guilty pleas during a short hearing on Wednesday.
The Ghanaian midfielder, who now plays for Spanish club Villarreal, faces trial under a High Court judge at the same court on November 2 next year.
Partey was first arrested in July 2022, though he was not named at the time and continued to play for Arsenal while investigations were ongoing.
More to follow…