Kyle Walker, Conor Gallagher and Ivan Toney are among other high profile names to miss out after being involved in the June camp, when England underwhelmed with a 1-0 win away to Andorra before suffering a shock loss at home to Senegal.
Cole Palmer, Jude Bellingham, Bukayo Saka and Levi Colwill are out with injury, while Trevoh Chalobah is also absent this time.
However, Tuchel has handed first senior call-ups to Elliot Anderson and Djed Spence, while other eye-catching youngsters Tino Livramento and Adam Wharton are involved too.
Senior figures Marc Guehi, John Stones, Jarrod Bowen and Ollie Watkins all return after missing the previous camp.
There is no recall for Jack Grealish, Harry Maguire or Phil Foden.
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England squad in full
Goalkeepers: Pickford, Henderson, Trafford
Defenders: Burn, Guehi, James, Konsa, Lewis-Skelly, Livramento, Spence, Stones.
Midfielders: Anderson, Gibbs-White, Henderson, Rice, Rogers, Wharton.
Attackers: Bowen, Eze, Gordon, Kane, Madueke, Rashford, Watkins.
Analysis: Anderson and Wharton to battle for midfield spot?
Sky Sports News’ Rob Dorsett:
“We expected there to be some significant headlines in this squad because the feeling I get is this is Thomas Tuchel stamping his authority and picking what he believes are the more serious names as he builds up to next summer’s World Cup. Gone is the experimentation of the early stages.
“The big headline inclusion is Elliot Anderson, who wins his first senior call-up. I was told Tuchel was thinking of calling him up for his June camp but wanted him to go with the U21s to the Euros and get some much-needed tournament experience.
“Another big headline is a first senior call-up under Tuchel for Adam Wharton, the Crystal Palace midfielder who we weren’t sure Tuchel fancied. He didn’t go to the U21 Euros but we know Tuchel is desperate for defensive midfield options. It looks like an interesting battle between Anderson and Wharton in this camp as to who gets the nod there.”
England’s upcoming fixtures
England vs Andorra – 5pm kick-off, September 6 – World Cup Qualifier
Serbia vs England – 7.45pm kick-off, September 9 – World Cup Qualifier
England vs Wales – 7.45pm kick-off, October 9 – Friendly
Latvia vs England – 7.45pm kick-off, October 14 – World Cup Qualifier
England vs Serbia – 7.45pm, November 13 – World Cup Qualifier
Albania vs England – 5pm kick-off, November 16 – World Cup Qualifier